Abstract: Rapid emergence of antibacterial resistance is well documented as a serious problem worldwide. This situation shows that the potencies of prevalent antibiotics are decreasing steadily. This situation implies the need for searching new antimicrobials to replace with invalidated ones or use in antibiotic rotation programs. In a four years study, from 1,300 soil Actinomycete isolates collected from different localities of Kerman, Hormozgan, Sistan and Baloochestan, south and south east Provinces of Iran, Streptomyces sp. isolate No. 419 showed widest antibacterial activity. The active principle named as Broadspectrim. It showed antibacterial activity against wide range of G+ and G- bacteria as Bacillus anthracis, Bacillus subtilis, Citrobacter diversus, Citrobacter freundii, Corynebacterium diphtheriae, Enterobacter sp., Escherichia coli, Klebsiella pneumoniae, Micrococcus luteum, Proteus vulgaris, Proteus rettgeri, Proteus mirabilis, Proteus morganii, Pseudomonas fluorescens, Pseudomonas aeruginosa, Pseudomonas syringae pv syringae, Salmonella Para Typhi A, B, C, D, Salmonella typhi, Sarcinia sp., S. marcescens, Shigella dysentery, S. flexneri, S. sonnei, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Vibrio cholera Eltor (INABA) and Xanthomonas sp. but Shigella flexneri and Staphylococcus albus were resistant to it.