Biotechnology

Year: 2003 | Volume: 2 | Issue: 1 | Page No.: 1-7
DOI: 10.3923/biotech.2003.1.7

Optimization of Various Parameters for the Production of Glucose Oxidase from Rice Polishing Using Aspergillus niger

Hafiz M. Hamid, Khalil-ur- Rehman, M. Anjum Zia and M. Asgher

Abstract: The enzyme glucose oxidase was produced by fermentation technology, using Aspergillus niger as fermentation organism and rice polishing as substrate. Maximum production of enzyme (3.42 U mL^-1) was recovered at substrate level of 2% after 36 h of submerged fermentation. The pH for the optimal production of enzyme was found to be 4. Addition of salts such as urea (0.3%), CaCO₃ (0.04%) and KH₂PO₄ (0.6%) into the fermentation medium enhanced enzyme production while MGSO₄.7H₂O was found to inhabit microbial growth and glucose oxidase production by A. niger.