Abstract: Background and Objective: Turfgrass resistance to heavy traffic and wear damage is one important quality in turfgrass. Since it differs within species, some wear resistance tests have been developed to further differentiate turfgrasses but they are more laborious and require longer time. Therefore, the aim of the study was to build an efficient tread loading system and evaluate its load condition on 3 commercial turfgrass cultivars: Zoysia matrella ‘Emerald’, Zoysia japonica ‘Meyer’, Cynodon dactylon×Cynodon transvaalensis ‘Tifway’ and a new Zoysia matrella cultivar ‘Wakaba’. Materials and Methods: A multiple tread loading system was constructed to accommodate 32 grass trays. Treading test was conducted on 4 turfgrass cultivars and the number of erect stems, stolon dry weight and green turf coverage were analyzed. Results: The newly registered cultivar ‘Wakaba’ had the highest number of erect stems and green coverage in both treading and non-treading (control) tests as compared to zoysiagrasses ‘Emerald’ and ‘Meyer’, while bermudagrass ‘Tifway’ least performed in all tests. Conclusion: It is concluded that tread loading system is efficient to analyze treading damage for multiple turfgrasses in a short period of time. In addition, the new cultivar Zoysia matrella ‘Wakaba’ has high resistance to treading and utilization of this cultivar is recommended.