Abstract: Background and Objective: The hydrophobicity and electron donor-electron acceptor properties of wood are considered an important parameters for microbial adhesion and wood degradation. Thus, the physicochemical properties of various wood species before and after treatment with Mentha pulegium, Rosmarinus officinalis and Cananga odorata essential oils were assessed. Materials and Methods: The hydrophobicity, electron acceptor (γ+) and electron donor (γ‾) properties of untreated and treated wood were determined using contact angle measurement. Results: All wood species tested have an electron donor character γ‾ higher than electron acceptor character γ+ and were characterized as having hydrophobic character except dibetou and beech woods which exhibit a hydrophilic character. The degree of hydrophobicity has decreased considerably using Mentha pulegium essential oil with values of water contact angles varying between 14.80±0.06 and 34.20±0.45°C followed by Cananga odorata and Rosmarinus officinalis essential oils with values of water contact angles ranged from 27.70±1.54° to 49.80±0.45° and from 37.00±0.35° to 58.90±0.45°, respectively. Conclusion: In this study, the hydrophobicity and the electron donor-electron acceptor properties have changed after treatment of wood species surfaces with the three essential oils.