Abstract: This study was carried out to create a new method of transplanting for various types of vegetables. An automated transplanter has been designed, developed and tested to be integrated with the main Gantry System in a greenhouse. The method was used by using the latest concepts using the jiffy peat pot (biodegradable). A Cartesian Configuration was used for the seed transplanter movement system which operates in a 3-axis format. The Auto CAD 2002 software was used to develop a 3D concept design of the proposed transplanter. The transplanter used electricity as its power supply. This machine consisted of a quick attach 3 point hitch, an X-axis module, a Z-axis module, an auger, a pot tray, a gripper and a watering unit. The transplanter operated automatically using a Graphical User Interface developed by Visual Basic 6.0 software. A stepper motor and a DC motor were used to drive the axes module. It was configured to integrate with the control system software which was developed by using FP WIN GR software and then downloaded to the Nais FP2 PLC, as hardware of the system.