Abstract: The high incidence of Campylobacter diarrhea represents a significant public health hazard. A total of 400 internal organs of either apparently healthy or diseased chicken were investigated for campylobacteriosis. The incidence of Campylobacter was higher in case of fecal samples, followed by fecal samples then duodenum samples. The highest recovery rates were obtained during summer season followed by the winter and spring, respectively. Campylobacter isolates were confirmed to be C. jejuni or C. coli using MapA and Col 2, 3 primers. The prevalence of virulence (cadF, flaA, ceuE) and CDT toxin (cdtA, cdtB, cdtC) encoding genes in C. jejuni and C. coli isolated from chicken illustrated that, 100, 78.6, 50 and 35.7% of C. jejuni isolates showed amplified fragments of ceuEj, cadF, cdtabc and flaA genes, respectively. Among the C. coli isolates 100% showed amplified fragments of ceuEc, no isolates had cdtabc genes, while 83.3% of C. coli isolates showed amplified fragments for cadF and flaA genes, respectively. Antibiotic resistance and plasmid virulence factors of Campylobacter were also estimated and all C. jejuni and C. coli isolates were totally susceptible to amikacin and tobramycin. Meanwhile high rate of antimicrobial resistance recorded may pose serious danger to the public health.