Abstract: The present study was performed to investigate the effect of dietary levels of protein, energy and Lys on growth performance and carcass traits of growing Japanese quails during 1-5 weeks of age. A factorial design 3×2×2 arrangement was performed including three levels of crud protein (CP; 22, 23.5 and 25%), two levels of metabolizable energy (ME; 2900 and 3000 kcal ME kg-1) and two levels of total lysine (Lys; 1.3 and 1.45%). Live Body Weight (LBW) at 3 weeks of age and Body Weight Gain (BWG) during 1-3 weeks of age were significantly (p≤0.01) maximized when chicks were fed 22% CP compared to those received 23.5 and 25% CP. LBW at 3 and 5 weeks of age as well as BWG through 1-3 and 1-5 weeks of age were statistically (p≤0.05) decreased with increasing dietary energy from 2900-3000 kcal ME kg-1 diet. LBW at 3 weeks of age and BWG during 1-3 weeks of age were maximum when chicks were fed 1.45% Lys compared with group received 1.3% Lys level. Interaction between protein and energy levels significantly (p≤0.01) affected LBW at 3 weeks of age and BWG through 1-3 weeks of age. Also, LBW at 5 weeks of age and BWG through 1-5 weeks of age were statistically (p≤0.05) influenced by the interaction between ME and Lys levels. Increasing dietary ME level from 2900 to 3000 caused significant improvements in FCR at all studied ages except period 1-3 weeks of age. The present results did not show any significant effect on all carcass characteristics due to different dietary CP, ME and Lys or their interactions, except carcass percentage was higher in chicks fed diet contained 1.3% Lys. Also, giblet percentage was significantly (p≤0.01) affected by energy levels and the interaction among dietary CP, ME and Lys levels. Moreover, dressing percentage was significantly (p≤0.01) influenced by CP and Lys levels. It can be concluded that, diet formulated in 22% CP, 2900 kcal ME kg-1 and 1.3% Lys can adequately ensure the growth performance in Japanese quails.