Abstract: The present work was conducted to evaluate the effect of prophylactic treatments using an ionophore anticoccidial (lasalocid sodium, 100 ppm) and a prebiotic (beta-glucans and oligosaccharides, 0.5 ppm) on coccidiosis and immune reactions against it in experimentally infected broiler chickens. A total of 120 chicks were equally divided into four groups. The first group (A) served as control and received the basal diet with no additives, while the other groups (B, C and D) served as tests and their birds were given the basal diet supplemented respectively with an anticoccidial (Avatec®), a prebiotic (Alphamune®) or a combination of them. Coccidiosis was induced in broiler chickens using a mixture of E. acervulina and E. maxima at the 14th day of age. By the end of the experimentation, better health condition and production performances were recorded in birds of the last group (D), in which a decrease in coccidia replication and lesional scores were found in association with low oocysts shedding kinetics and a stimulated immune system (increased lymphoid organs weights, indices and a bigger number of lymphocytes). Our findings confirm the effectiveness of the prebiotic-anticoccidial combination in the control of coccidia infections in broiler chickens.