Abstract: This study was aimed to assess the effect of supplementing gum Arabic in the basal diet of the laying hens (Historic strain of single comb white leghorn) on egg production and quality. Seventy two White Leghorn laying hens (20 weeks of age) were randomly divided into four groups (A, B, C and D), 18 birds in each. Group A received the basal diet and group B to D received the basal diet supplemented with 5, 10 and 15% (w/w) Acacia senegal gum, respectively. Birds were allowed free access to feed and water and the feeding trial lasted for 8 weeks. Gum Arabic (15%) increased (p<0.05) feed intake and egg shell thickness by 5.98 and 31.58%, respectively as compared to the control. The significant (p≤0.05) increase in albumin, Ca and P was also noticed in blood serum and egg yolk. Increasing the ratio of the gum Arabic (from 5-15%) in the basal laying hen diet significantly (p≤0.05) reduced serum cholesterol in a gradual manner and consequently eggs with lowered yolk cholesterol were obtained.