Abstract: Background and Objective: The discovery of antibiotics helped in the combat against bacterial infections that cause various diseases, but infectious bacteria formed a resistance against these drugs due to their misuse, there has been a growing interest in the study and use of medicinal plants in the world. Eleven plant extracts were screened for antibacterial efficacy and synergistic effect against Escherichia coli and Staphylococcus aureus in this study. Materials and Methods: Ethanolic and aquatic extraction were carried out in the microwave-assisted method. The antimicrobial activities were assessed by disc and agar well diffusion technique, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The synergistic assay was carried out by mixing Coriandrum sativum extract with Artemisia monosperma extract and Cuminum cyminum extract with Ocimum basilicum extract. Results: The findings demonstrated that S. aureus was less susceptible to the plant extracts employed than E. coli. Except for Syzygium aromaticum, which demonstrated a broad zone of inhibition on the bacteria and a confluence between the ethanol extract findings and those of the aquatic extract in both the disc and well diffusion procedures, aqueous extract inhibits more bacteria than ethanolic extract. When compared to Syzygium aromaticum, Hibiscus sabdariffa displayed the lowest MIC value in aqueous extract when compared to ethanolic extract. MBC values against E. coli and S. aureus for the majority of plant species in each ethanolic and aquatic extract were >200 mg mL–1. Conclusion: Furthermore, the current study looked at the usefulness of synergistic effects of various extracts against pathogenic bacterial isolates. The findings of employing the disc and well diffusion method and a low MIC value demonstrated a mild synergistic impact of various plant extracts.