Abstract: Background and Objective: The plant species, Paederia linearis, Paederia foetida and Rotheca serrata have long been used as a traditional medicine for male vitality. So, they were investigated for data that supports their traditional safe uses including phytochemicals, investigating possible mechanisms and toxicity testing of their range of medicinal properties. Materials and Methods: Gas Chromatography-Mass Spectrometry (GC-MS) and High-Performance Liquid Chromatography (HPLC) was were used for phytochemical analysis. Toxicity testing including cytotoxicity and genotoxicity as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and comet assays were investigated. Results: The results of this research were shown the following: Major phytochemicals were phytol and squalene in P. linearis, phytol in P. foetida, while other compounds were found in small amounts and most of them were unknown substances (whereas, Rotheca serrata was previously reported to contain phytol and oleamide). The L-dopa was found in the hexane extracts of all three plants. The rice bran oil extracts of P. linearis and P. foetida which can be edible showed higher L-dopa amounts and concentrations than the hexane extracts. The MTT assay indicated no toxicity at the cell level, but the comet assay showed significant (p<0.01) toxicity at the DNA level in all three plant extracts. However, when evaluated for human dosing, they can be safely consumed orally. Conclusion: These investigated compounds support the three studied plants having long been used traditionally in Thailand in a fresh form with several active phytochemicals including L-dopa.