Abstract: Background and Objective: Shallot c.v. lembah palu is one of the main commodities of Central Sulawesi and is a raw material for the fried shallot processing industry. The research aimed at finding out the initiation of shallot shoots c.v. lembah palu on various concentrations of seaweed (Caulerpa sp.) extract and benzyl amino purine in vitro. Materials and Methods: The research was designed as a factorial completely randomized design, consisting of two factors. Each factor has three levels. The first factor was the concentration of seaweed extract 0, 20 and 40%. The second factor was the concentration of BAP 0, 2 and 4 ppm. Each treatment combination was repeated three times. The effect of the treatments was analyzed using analysis of variance and the mean difference between the treatments was analyzed with Honestly Significant Difference (HSD) at the level of 5%. Results: The results show that there is significant effect interaction between concentration treatment of seaweed extract and BAP on the number and length of the shoots on 6, 12 and 18 days after planting as well as the time of shoots emergence. The interaction between without BAP treatment and 20% of seaweed extract gave the highest number and the longest shoots which is significantly different from other treatments. Conclusion: The addition of both seaweed extract and BAP on higher concentration (40% and 4 ppm) raised the shoots faster, yet it was insignificant different with the treatment of 20% of seaweed extract without BAP.