Abstract: Background and Objective: Salicylic Acid (SA) is a plant hormone that plays a key role in regulating many physiological processes in plants, including seed germination, plant growth, photosynthetic efficiency, crop yield, flowering and senescence. This study aimed to investigate the physiological responses of Chrysanthemum variety “Mai Vang” to different concentrations of SA (0, 0.5, 1.0, 1.5 and 2.0 mM). Materials and Methods: The Chrysanthemum were treated by various concentrations of SA (0.5, 1.0, 1.5 and 2.0 mM), water treatment (SA0) was considered as control. The experiment conducted in a complete randomized design with a total of five treatments, each plot consisted of 15 plants. Duncan’s multiple range test was being used for statistical analysis at the 5% level of significance (p = 0.05). Results: The SA treatments affected the content of photosynthetic pigments, proline and malondialdehyde (MDA) in leaves as well as anthocyanin in florets. Generally, SA treatments at a concentration of 0.5 and 1.0 increased the content of chlorophylls and carotenoids while decreasing the content of MDA compared to water treatments. In contrast, SA1.5 and SA2.0 treatments decreased the content of these pigments while enhancing the accumulation of MDA compared to control. All SA treatments displayed higher content of proline in leaves than control. Also, plants treated by SA at 0.5 and 1.0 mM exhibited higher maximum quantum yield of photosystem II (Fv/Fm) value than untreated and 1.5 and 2.0 mM of SA treated ones. Conclusion: The present study provides further insight into the effectiveness of SA on the physiological characteristics of Chrysanthemum “Mai Vang”. Especially, SA at 1.0 mM had the maximum positive effect on the content of photosynthetic pigments, proline, anthocyanin and Fv/Fm index. Moreover, SA1.0 treatments exhibited a significant decrease in MDA content compared to control.