Abstract: Background and Objective: Corchorus olitorius is a leafy vegetable and medicinal plant rich in antioxidants and also cultivated worldwide for its stem fibers. This work studied the influence of micronutrients fertilization on growth and antioxidant activity of this plant. The study aimed to help improvement of this crop yield as well as study the impact of fertilization on its characteristic antioxidant activity. Materials and Methods: In this work, micronutrients fertilization by Cu, Zn, Ni and Fe were studied for their effect on fresh weight, stem length, leaf number and leaf areas of C. olitorius. Antioxidant activity was estimated by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay. A number of non-enzymatic antioxidants were estimated including free and wall-bound phenolics, total flavonoids, total anthocyanins and ascorbic acid. Results: The results recorded increased growth and reduced antioxidant activity as a result of all metal treatments, except Ni. Iron treatment resulted in the best growth and least antioxidant activity. After a second dose of the tested metals, growth rate decreased with increasing metal concentrations and this was accompanied by elevated antioxidant activity. These results record the positive impact of micronutrient fertilization on crop production of jute. Conclusion: However, the negative impact on its antioxidant value suggests a limitation on micronutrients fertilization of this plant if cultivated for consumption as a vegetable or for medicinal purposes.