Abstract: Background and Objective: In developing wheat varieties adaptive to lowlands and drought stress, one of the screening methods are by hydroponic culture. Specific development related to the character screened and selection environment is required for the selection process to be more stable and accurate. The study aimed to identify the character and environment of hydroponic screening selection and to select the mutant strains of tropical wheat that are adaptive to drought stress. Materials and Methods: The study consisted of two experiments, namely screening of 21 wheat mutant genotypes and 4 comparison varieties on hydroponic culture and its validation on soil potting media for the yielding character. Four concentrations of Polyethylene Glycol (PEG) (0, 5, 10 and 15%) and two levels of water status, 80 and 60% of field capacity were used in the hydroponic culture screening test and on soil media, respectively. The data obtained were analyzed using analysis of variance and multivariate analysis. Results: Results showed that a concentration of 10% PEG was the best PEG concentration in selecting wheat genotypes adaptive to drought stress in hydroponic cultures. The weight character of 100 seeds and the number of grain per panicle are secondary characters that support productivity. The selected selection index was able to select seven genotypes that are adaptive to drought stress. Conclusion: These results concluded that the screening of drought on wheat through hydroponic culture was considered to be able to replace the screening of wheat drought on the soil in pots.