Abstract: Background and Objective: Cytogenetic and kinship of seven local corn cultivars from Kisar Island, have different chromosome characteristics. The research was aimed to analyze the cytogenetic and kinship of seven local corn cultivars from Kisar Island, Southwest Maluku based on the chromosome characteristics. Materials and Methods: The samples of the study were the seed of seven local corn cultivars. Chromosome analysis was conducted with the squashing method. Cytogenetic traits were observed namely chromosome number, size and shape using Micromeasure 3.3 software and Karyotype Smart Express and the identification of the corn cultivars kinship was performed using cluster analysis. Results: Seven Kisar corn cultivars have the same chromosome number (2n = 20). The smallest size of merah delima tongkol coklat (69.49 μm), the largest Kuning genjah (120.12 μm). The composition of the corn cultivar karyotype is similar, except for merah darah (chromosomes 14 and 15). The closest relationship between pulut and putih (c.i 0.004) while the farthest between kuning genjah and merah delima tongkol coklat (c.i 3.214). Conclusion: Also, corn has the potential to be developed into higher quality plants and needs to be supported by cytogenetic information, namely chromosome analysis. It is expected that karyotype research on the seven Kisar corn cultivars can provide information for the community to develop breeding efforts for the seven Kisar corn cultivars.