Abstract: Background and Objectives: Marathwada region shows vertisols type soil and classified as deep black soils, medium black soils and coarse shallow soils. This paper describes the status of pteridophytes and association of arbuscular myorrhizal fungi (AMF) from Marathwada region of Deccan Plateau Zone of India. Materials and Methods: Pteridophytic plant species were collected from Nanded, Hingoli, Latur, Parbhani, Jalna, Aurangabad, Beed and Osmanabad area during July, 2014 to September, 2016 and assessed the AM fungal infection. Pteridophytic plants were surveyed, recorded, photographed and identified with the help of different flora. The rhizosphere soil and root samples were collected and analyzed AMF (%) root colonization and types. Results: Pteridophytic 10 plants species belonging to 5 different families were surveyed viz., Actinopteris radiata (Pteridaceae) Adiantum caudatum (Pteridaceae), Adiantum lunulatum (Pteridaceae), Adiantum capillus-veneri L. (Pteridaceae), Cheilanthus farinosa (Forssk.) Kaulf (Pteridaceae), Equisetum hyemale L. (Equisetaceae), Marsilea quadrifolia (L.) (Marsileaceae), Nephrolepis exaltata (L.) (Lomariopsidaceae), Ophioglossum vulgatum L. (Ophioglossaceae) and Pteris vittata L. (Pteridaceae) from 8 different district. Present results were varied in Root Colonization (RC) from 06.25-50%, root length colonization (Rlc) was ranged from 1.56-14.28% and types of root colonization were hypal (H), vesicles (V), Arbuscle (A) and Dark septate endophytes (DSE). Conclusion: Study concluded that, among studied species; Nephrolepis exaltata and Marsilea quadrifolia were found dominant AMF root colonization was found in all collected plant species and AMF infection was observed more in O. vulgatum while less in P. vittata.