Abstract: Background and Objective: Celastrus paniculatus Willd. (Celastraceae) is a large, woody climbing shrub commonly known as ‘Jyotishmati’, "Intellect tree" or ‘Bitter sweet’ is an important medicinal plant in India. Due its high pharmaceutical applications, this species has been over exploited and is now considered as endangered species. The present investigation was focused on development of an efficient shoot induction protocol for the large scale production of Celastrus paniculatus. Materials and Methods: The pathological screening of in vitro propagated progenies was done to ensure their disease free nature. Murashige and Skoog (MS) basal medium supplemented with varying combination of growth regulators such as Ammonium nitrate (NH4NO3), Benzyl Amino Purine (BAP) and Kinetin (Kin) with a concentration ranging from 0.5-3.0 mg L–1 for shoot induction. To detect the presence of any microbial growth, the in vitro derived clones with 0.5 mm in size were transferred into Potato Dextrose Agar (PDA) or Plate Count Agar (PCA) medium and the visual observation for the detection of endogenous and exogenous fungal and bacterial growth. A random sampling of 50% of in vitro propagated plants listed for microbial contamination. The results were recorded and expressed as Mean±SD for all the experiments. Results: The maximum number of shoots was induced in MS medium with 1.5 mg L–1 of BAP, it was 4.67±1.58 shoots. Subsequent culture on medium with 1.0 mg L–1 of BAP facilitated rapid multiplication and a mean of 30.52±2.64 shoots were developed. The developed shoots were healthy and a height of approximately 8 cm. Conclusion: The developed protocol was successfully employed for the large scale production of this endangered species. The pathological screening revealed the disease free nature of the developed shoots.