Abstract: Objective: The purpose of the study was to investigate the effect of planting material storage method and storage duration on stake characteristics and germination percentage in 3 cultivars of cassava under rain-fed conditions. Methodology: The study was laid out in a split-split plot design with 3 replications. The main plots were comprised of 4 storage durations of planting material: 0, 15, 30 and 45 days after the planting material was harvested. The sub-plot treatments consisted of 3 storage methods of planting material: (1) Placing outdoors by digging a hole approximately 15-20 cm deep, (2) Placing under the tree shade and (3) Placing outdoors covered with hemp sacks. The sub-sub-plot treatments consisted of 3 cassava varieties: Kasetsart 50, Rayong 7 and 72. Results: The results illustrated that among cultivars, significant differences were observed in the following characters: Percentage stake moisture, total K content after storage duration for 15 and 45 days, total P and K content after storage duration for 30 days, percentage germination and percentage survival. Rayong 72 had the greatest percentage stake moisture and percentage total K content after storage duration for 15 and 45 days and percentage survival rate after storage duration for 15 days (98.15%). Planting material storage methods significantly affected percentage stake moisture and percentage total K content after storage duration for 45 days. The storage methods of placing outdoors by digging a deep hole of approximately 15-20 cm and covering with hemp sacks resulted in greater percentage stake moisture (70.51-71.37 and 0.94-1.11%, respectively) than the storage method of placing under tree shade. For storage duration, the result indicated that 45 days of storage reduced the percentage germination and percentage survival rate. Conclusion: The storage duration of planting material should not exceed 30 days with placing outdoors covered with hemp sacks and placing under the shade of a tree were superior to have fast and higher germination and survival percentage.