Abstract: The study was carried out in three locations (a total of 7 environments) of Northern Ethiopia from 2011-2013 cropping seasons and thirteen sesame genotypes were evaluated. The objective of this study was to determine the magnitude of G×E interaction and stability of sesame genotypes. The experiment was laid out in randomized complete block design with three replications and a total plot size of 14 m2. The Additive Main effects and Multiplicative Interaction (AMMI) model for grain yield detected significant effects of the genotypes (37.3% Sum of Squares (SS)), environments (29.5% Sum of Squares) and Genotype×Environment interaction (25.9% SS). The model also extracted five significant Interaction Principal Component Analysis (IPCA) with a total of 96.9% SS and 90.3% corresponding degrees of freedom. Acc-034 (G4) (926.8 kg ha-1) followed by Acc # 031 (G1) (895.1 kg ha-1) had the highest average yield which was much greater than the grand mean (742.9 kg ha-1) and declared as area specific adapted genotypes. Based on the magnitude of the IPCA1, Yield Stability Index (YSI) and Sum of Interaction Principal Component (SIPC), Setit-1 (G12) with greater yield (832.7 kg ha-1) than the grand mean was declared as widely adapted genotype. Environments E1, E2 and E4 were unfavorable environments while E5, E6 and E7 were favorable environments and E3 was moderately favorable environment for most of the sesame genotypes.