Abstract: Among the major root and tuber crops, anchote is a potential crop produced in West Wollega zone of Ethiopia. It serves as a food, cultural, social and economical crop for the farming communities. Due to the lower attention given to the research and development of anchote, there is no variety so far developed and released. Ten promising anchote accessions were tested at Jimma and Ebantu from June 2010 until October 2010 to determine agronomic performance of the accessions. The experimental design was a randomized complete block design with three replications. The results indicated that yield and yield components were significantly (p<0.05) higher for the majority of the accessions under Ebantu than Jimma condition. The highest total biomass, 19.13 kg per 4 m2 plot, was obtained at Ebantu from accession 223098 while the maximum total biomass at Jimma was only 11.69 kg per 4 m2 plot that was obtained from accession 223087. Almost all accessions took longer average time (128 days) to reach 90% maturity under Ebantu condition than Jimma which took 109 days on average. The maximum storage root yield (76.45 t ha-1) was observed for an accession No. 223098, under Ebantu condition, while, the lowest yield was obtained from accession No. 240407 (51.54 t ha-1) under Jimma condition. The highest mean dry matter (30%) was obtained under Ebantu condition for almost all accessions. On the other hand, the lowest mean dry matter (20%) was obtained under Jimma condition. From this study the six accessions 223109, 223087, 223098, 223096, 90802 and 229702 produced better storage root yield, high dry matter content, high biological yield across the two environments indicating a good performance and adaptation. Therefore, these accessions are suggested to farmers in areas of Jimma, Ebantu and with other areas of similar agro-ecological zones.