Abstract: Estimates of variability and presence of genetic interaction of protein and micronutrient (Iron, Copper, manganese, Zinc) characters were analyzed in segregating generations derived from a cross between BPTxHPR 14 of rice. Total Grain Protein Content (GPC) showed high heritability coupled with a moderate genetic advance in both generations. From F4 to F5, heritability estimates showed consistency indicating fixation of loci controlling protein content. According to estimated coefficients of skewness and Kurtosis indicated the presence of duplicate gene interactions in the expression of total grain protein content, copper and iron content whereas complementary gene interaction appears to be absent in the expression of zinc and manganese content. The results indicate the mild selection can be employed for traits like protein content, copper and iron content in segregating generation. The method of using skewness and kurtosis for detecting additive epistasis is very simple and can be used for crop improvement.