Abstract: The aim of this study was to find the effect of fungicide of Foenicolom vulgare, Carum carvi and Carum copticum essential oils in the control of fungal pathogen Botrytis cinerea, the casual agent of grey mould disease of cucumber (Cucumis sativus L.) in vitro and in vivo conditions. At first experiment, antifungal effects on essential oils were carried out with Solution Method (SM). In second experiment, fruits were infected artificially by B. cinerea spore and then treated by different concentrations of these essential oils. In vitro and in vivo experiment were conducted in a completely randomized factorial design with two factors; including five concentration treatments (0, 200, 400, 600 and 800 μL L-1) as first factor and three using essential oils as second factor. The results of in vitro showed that C. carvi essential oil had the most of fungicidal effect in comparisons with other essential oils. The growth of B. cinerea was completely inhibited by C. carvi, C. copticum and F. vulgare essential oils at relatively low concentrations (400 and 600 μL L-1). The results of in vivo experiment showed that C. copticum, F. vulgare and C. carvi essential oils at all applied concentrations inhibited B. cinerea growth on cucumber fruits completely in comparison to controls. Also these essential oils at all concentrations showed positive effects on some fruit quality characteristics e.g., titrable acidity, Total Soluble Solid, Ascorbic acid, pH and weight loss and these essential oils inhibited of infection fruits to grey mould and increased life storage fruits.