Abstract: This study was conducted to enrich the soil nutrients and plant growth using Glomus fasciculatum and Rhizobium inoculation along with input of N and P fertilizers. The entire study was designed in low nutrients available soil collected from Marikundu (Theni District, Tamilnadu). The supplemented nitrogen with Rhizobium and Glomus fasciculatum showed increased biomass (0.439 g plant-1), total nitrogen content (38.13 mg N g-1 dry plant) and phosphorus content (5.16 mg P g-1 dry plant) in dual inoculation at 200 mgN kg-1 of soil). Similarly, supplemental phosphorus in combination with dual inoculation significantly increased the biomass, total nitrogen content and phosphorus content followed by Rhizobium and Glomus fasciculatum inoculated plants. The nitrogen content was found to be higher in all dose rates of dual inoculated plants, specifically higher at 50 mg P kg-1 of soil (22.83 mg N g-1 dry plant). Dual inoculation with Rhizobium and Glomus fasciculatum increased the nodule nitrogenase activity (36-213%), dry matter yield (156-279%), total nitrogen content (12-159%) and total chlorophyll content of leaves of Acacia mellifera (125-395%) compared to the uninoculated control or single inoculation with either Rhizobium or Glomus fasciculatum alone. This study considered the impact of triple interaction involving Rhizobium AcM05, Glomus fasciculatum and Acacia mellifera on soil nutrients, soil enzymes and microbial dynamics.