Abstract: Garden croton, an ornamental shrub or tree with diverse beautifully colored leaves, shapes and sizes, is used as informal hedge or screen for landscape, interiorscape and bouquet. This study, investigated the effects of cigarette tobacco extract on plant mutation, root regeneration and proliferation in a plant-plant interactive hydroponic assay using two well known reciprocal mutant cultivars (Guinea broad leaf and Guinea narrow leaf) of garden croton. The results showed that 1x10-(4-8) logarithm dilution of a standard teacup (270 mL) cigarette tobacco-infusion enhanced root regeneration, elongation and proliferation above control level. Higher concentrations of extract inhibited root elongation and proliferation. Undiluted tobacco infusion extract (100%) delayed root initiation until four weeks. Overall, the extract enhanced root elongation and proliferation better in the Guinea broad leaf than the Guinea narrow leaf cultivar. There was no sign of induced mutation in the plant cultivars by the extract.