Abstract: Rhynchostylis gigantea (Lindl) Ridl is native Thailand. It was difficult to propagate under natural conditions. Tissue culture technique is a way that had high potential to solve this problem. This experiment Vacin and Went was conducted to test modified medium for R. gigantean. The modified media were applied as the combination of scale and liquid fertilizer. In addition modification medium, those media were mixed with pupa powder, bamboo-charcoal, potato, sweet potato, unpolished rice, banana pulp and coconut water as supplementary compounds. The modified media were used to replace synthetic chemicals that were used to prepare tissue culture media. The result showed that, the formulation of modified-media; 2 g L-1 of bamboo-charcoal, 5 g L-1 of pupa powder, 50 g L-1 of banana pulp, 50 g L-1 of unpolished rice and 150 mg L-1 of coconut water showed the highest of plant growth and development, plant height (8.05 cm), fresh weight (1.753 g), number of leaf (5 leafs) and root number (3.9 roots). After transplanted to green house for 6 weeks, all of modified media did not have statistically significant of the number of survival plants and chlorophyll content. Thus, the natural modified media had the potentiality to be applied as R. gigantea cultural media to replace the synthetic chemical media.