Abstract: An experiment was conducted to study the effect of different rates of nitrogen (N) fertilizer with two types of bio-fertilizers (0, 125, 75, 225, 125 plus Super Nitro and 125 kg N h-1 plus Nitroxin bio-fertilizer) and two cultivars (Super Chief and Super Beita) on growth and yield of tomato, at a field Andimeshk, Khuzestan Iran, during 2006. The experiment design was randomized complete block design arranged in split plot, with three replications. During of study, were measured some of vegetative and reproductive characteristics such as seedling emergence time, plant height, leaf number, leaf number below the inflorescence, flowering time in the first inflorescence, flowers and fruits number per the first inflorescence, fruit mean weight, fruit number per plant and fruit yield. Results indicated that there were significant differences between N level and most of characteristics such as plant height, leaf number, fruit number per the inflorescence, fruit number per plant, fruit mean weight and fruit yield and bio-fertilizers affected the growth parameters such as seedling emergence time, plant height, leaf number, fruit mean weight and fruit yield but they have not significant effect on flowering time, flower and fruit number per the first inflorescence and fruit number per plant. Also there was significant differences between the cultivars in seedling emergence time, plant height, flowers and fruits number per the first inflorescence, fruit mean weight and fruit yield. The maximum yield was accomplished when Super Beita cultivar received 225 kg N h-1.