Abstract: The investigation was done to find out the tissue culture potentiality of the local rice (Oryza sativa L.) variety Topa, cultivated mainly in Kishoregonj, the district of Bangladesh. In this present study, callus induction, callus growth rate and indirect regeneration potentiality of the variety was examined. One hundred percent callus induction efficacy was noted when dehusked mature seeds were cultured on MS media supplemented with 2.0 mg L-1 2, 4-D. After first successive subculture the highest callus growth rate (0.0791 ±0.017 g week-1) was observed under the best callus induction media. The highest regeneration response was recorded at treatment of 3.0 mg L-1 BA+0.5 mg L-1 NAA+0.5 mg L-1 Kn, which regenerated 80% shoot with an average of 3 shoots per explant.