Abstract: Three new low molecular weight glutenin subunits (LMW-GS) genes, designated LMWYN-1, LMWYN-2 and LMWYN-3, were isolated and characterized from Yunnan hulled wheat accession YN11 (Triticum aestivum subsp. yunnanense King). The length of complete nucleotide sequence of LMWYN-1, LMWYN-2 and LMWYN-3 were 1056, 960 and 897 bp, respectively. LMWYN-1 and LMWYN-2 encoded proteins with 330 and 299 amino acid residues, respectively, whereas LMWYN-3 was a putative pseudogene due to the presence of three stop codons in the coding region. All deduced amino acid sequences of the three genes were the LMW-m type according to a methionine at position 1 of the putative mature protein. In repetitive domain of LMWYN-1 two hydrophobic motifs PIIIL and PVIIL were observed. LMWYN-1, LMWYN-2 and LMWYN-3 were classified as groups 5, 2 and 8, respectively. Phylogenetic analysis showed that LMWYN-1 and LMWYN-3 were closely related to the genes on chromosome 1D and LMWYN-2 was homologous with the genes on chromosome 1B.