Abstract: This study was carried out to evaluate morphological traits of the grass pea landraces using a randomized complete block design with 3 replications at Research Farm of Isfahan University of Technology and evaluation genetic diversity of 20 grass pea landraces from various locations in Iran were investigated using 32 RAPD and ISJ primers at Institute of Agriculture of Biotechnology of University of Zabol. Analysis of variance indicated a highly significant differences among 20 grass pea landrace for the morphological traits. Average of polymorphism percentage of RAPD primer was 73.9%. Among used primer, 12 random primers showed polymorphism and a total of 56 different bands were observed in the genotypes. Jafar-Abad and Sar-Chahan genotypes with similarity coefficient of 66% and Khoram-Abad 2 and Khoram-Abad 7 genotypes with similarity coefficient of 3% were the most related and the most distinct genotypes, respectively. Fourteen primers out of 17 semi random primers produced 70 polymorphic bands which included 56% of the total 126 produced bands. Genetic relatedness among population was investigated using Jacard coefficient and Unweighted Pair Group Mean Analysis (UPGMA) algorithm. The result of this research verified possibility of use of RAPD and ISJ markers for estimation of genetic diversity, management of genetic resources and determination of repetitive accessions in grass pea.