Abstract: A field trial was conducted to assess the effect of different sources of fertilizers on the growth, cane yield and sugar recovery of promising sugarcane variety Larkana-2001, at Sugarcane Section, Agriculture Research Institute, Tandojam, Pakistan. The treatments included four fertilization sources (225-112-168 NPK kg ha-1, effective microorganism (EM) material, 25 t ha-1 farm yard manure (FYM) and 25 t ha-1 press mud. The chemical source of fertilizer (NPK) at the rate of 225-112-168 kg ha-1 proved to be more effective to produce significantly greater plant height and thicker cane girth, more number of tillers, better brix, higher sugar recovery and maximum cane yield ha-1. The crop fertilized with EM prepared material and 25 t ha-1 press mud ranked 2nd and 3rd for all the crop parameters. However, application of 25 t ha-1 farm yard manure was not much effective fertilizer source for crop growth and quality characters.