Abstract: The objective of this study was to evaluate the potential of F2 hybrids by comparing them with parents, commercial varieties and F1, s for yield, yield components and fibre quality. The genetic design was half diallel, consisting of five parents and additional five direct crosses were attempted to study inbreeding depression (CIM-496, MNH-554, FH-901, FH-945, LRA-5166, 10 F1 and 10 F2). The difference among genotypes were determined. The highest yielding parent was CIM-496 with 3521 kg ha-1 followed by MNH-554 with 3268 kg ha-1. While the variety FH-901 with 2391 kg ha-1 showed minimum yield among the parents. The cross combinations MNH-786 x VH-144, MNH-554 x LRA-5166 and CIM-499 x LRA-5166 showed minimum inbreeding depression i.e., -39.72, -27.85 and -22.72 for seed cotton yield, yield components and fibre traits than expected inbreeding depression i.e., 50.0%. General combining ability mean squares were significant for all traits and specific combing ability mean squares were also significant for all traits. The GCA effects were higher than SCA effects for all traits, which indicates that additive gene action is prevailing with dominant gene action for expression of these traits. The variety FH-901 was the best general combiner for the yield and yield components. It is concluded that F2 can be used for availing the heterosis after evaluating the proper cross combinations, be reduced.