Abstract: This research was conducted to study the effects of AMF (Arbuscular Mycorrhiza Fungi) on yield and root in relation with salinity tolerance index and mycorrhizal dependence on genotypes of barley. To this order, in green houstrial, 4 genotypes of barley (hull less and hull barley) harvested in lisimetry pools in Gorgan. Each plant species was either mycorrhizal fungus, Glomus intraradicaes or non-mycorrhizal and irrigated with 3 NaCl levels (0, 8 and 16 dS m-1). Data was analyzed with two factor (RCBD). Also, interaction between mycorrhiza and different genotypes of barley were studied. Analysis of variance indicated that at salinity levels and mycorrhiza inoculation, ratio shoot to root, spike weight and length, yield, number of seed, mycorrhizal dependencies and tolerance indices, were significant (p<0.05). Correlation coefficients among the traits showed that most of them had positive correlated with each other in this survey. There was no significant correlation among to root length with, ratio shoot to root, root weight, spike length, spike weight and seed number and root weight with ratio shoot to root (p<0.01 and 0.05). Single regression has done with Enter method that yield as dependent variable showed 92% variation of yield explained with WDSp/Yield and 64% with WDSp and 73% with RW. Also in arrangement variables such as WDSp/Yield, WSp and RW entered to regression equation stepwise method so that 98% variation of yield proved. Results showed that the inoculation of AMF affected on chlorophyll contents. In EC = 8 dS m-1, mycorrhizal dependency increased. High tolerance index was in EC = 8 dS m-1. Yield of barley with AMF increased at all salinity levels. The AMF adaptation has shown by this study may explain to increase the salinity tolerance of genotypes barley under salinity conditions.