Abstract: This study was applied to elucidate the taxonomic status and to evaluate genetic diversity of L. glaber Mill. populations distributed along 5 territories of Fayoum depression. This was carried out through scoring of 39 morphological characters, chromosome counts and isozyme electrophoresis. The diagnostic morphological characters and diploid chromosome number 2n = 12 elucidated that the collected accessions from Fayoum depression should be treated as L. glaber. Populations having higher genetic diversity measures were grouped in one cluster. These populations were collected from highly saline soils with imperfect to poor drainage insuring the tolerance of L. glaber to salinity and poor soil conditions. This verified the importance of L. glaber as a valuable genetic resource that can be used for improvement of L. corniculatus. More research was initiated in our lab to link salt tolerance trait with DNA marker.