Abstract: Distinction, Uniformity and Stability tests of the cotton advanced lines were performed by Nazilli Cotton Research Institute in Aegean Region of Turkey. Studies were conducted in three location of Aegean Region (Nazilli, Soke and Saraykoy), to evaluate the lint yield and fiber characteristics of 20 genotypes in 2005. The location x genotype interactions were significant for all characters except fiber length and fiber strength. Ozbek 142 S for lint percentage and Carmen for fiber strength were considerably the highest values for all locations. The most adaptable genotypes in yields were N/D-121 for Nazilli and Saraykoy, Ozbek 142 S for Soke.