Abstract: Pre-harvest sprouting seriously reduces durum grain quality and is considered as an important grading criterion in all market classes of wheat (Triticum sp.). In northern Iran, untimely rain and humid conditions at harvest time cause sprouting of kernels in spike. There are few researches in the literature on pre-harvest sprouting and related traits in durum wheat (Triticum turgidum L. var. durum). A two years study (2001 and 2002) was conducted to evaluate the genetic variation of sprouting resistance and related trait in the Iranian germplasm of durum wheat accessions. In 2001, a total of 532 accessions were planted under field conditions. Unreplicated samples of 10 spikes from each genotype were evaluated for spike sprouting and falling number under laboratory conditions. Using two screening methods, the 2001 study indicated that durum accessions had a similar range in sprouting score and sprouting index to the wheat check cultivars. The sprouting score for durum accessions ranged from zero to 10. Twenty-eight of selected accessions with high level of sprouting resistance were re-evaluated in a replicated study in the second year. Sprouting score and sprouting index measured in 2002 were correlated with those of 2001. According to the results of this study, there is considerable genetic variation in sprouting resistance in durum germplasm that can be exploited by breeding programs.