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Asian Journal of Plant Sciences

Year: 2006 | Volume: 5 | Issue: 1 | Page No.: 123-126
DOI: 10.3923/ajps.2006.123.126
Factors Influencing the Efficiency of Extraction of Polyphenols from Young Tea Leaves
K.M. Mariya John , Deepu Vijayan , R. Raj Kumar and R. Premkumar

Abstract: A study was conducted to overcome certain difficulties in the extraction of phenolic compounds from tea leaves. Concentration of the solvent, methods of extraction, acidification, temperature and purification are the limiting factors in extracting higher amount of polyphenols from tea leaves. The extraction solvent methanol (80 to 90%) in water with 3% hydrochloric acid gave promising results. Refluxing the sample with solvent for one hour and high-speed centrifugation yielded high quality of polyphenols. It was observed that polyphenol yield can be increased by repeated extraction with fresh solvent. The purity of the extract was highest in ethyl acetate treated with 2% of hydrochloric acid (98.04%). This was confirmed by running the extracted samples using HPLC.

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How to cite this article
K.M. Mariya John , Deepu Vijayan , R. Raj Kumar and R. Premkumar , 2006. Factors Influencing the Efficiency of Extraction of Polyphenols from Young Tea Leaves. Asian Journal of Plant Sciences, 5: 123-126.

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