Abstract: This research paper attempts to investigate the performance of Asparagus racemosus grown by the application of different forms and doses of nitrogen fertilizer at the Germplasm Centre (GPC) of the Fruit Tree Improvement Program (FTIP), Department of Horticulture, Bangladesh Agricultural University, Mymensingh during February to October, 2005. The treatments consisted of prilled urea and super granule urea at 0, 100, 200 and 300 kg N ha-1 concentrations. The experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. The number, length, diameter and both fresh and dry weight of tuberous roots were found higher with super granule urea than that of prilled urea. Root protein content was found to be 25.20% higher in super granule urea treated plants compared to prilled urea. The rates of nitrogen also had a significant effect on plant height, leaves number and number, length, diameter and both fresh and dry weight of tuberous roots when compared to 0 kg N kg ha-1 to the rest of the rates. Root protein content was 21.87, 12.5 and 14.06% higher than the control at the 100, 200 and 300 kg N ha-1 concentrations, respectively. Therefore, application of 100 kg N ha-1 as super granule urea was found to be sufficient for the sustainable production of tuberous roots of Asparagus.