Abstract: The effects of three Salvia species (S. officinalis, S. dominica and S. spinosa) on five bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella sp. Escherichia coli and Enterobacter sp.) were investigated. Also, the genetic variation among the Salvia sp. was determined. The three Salvia sp. showed different antimicrobial activity in different extract amounts against the five bacteria. Salvia officinalis and Salvia dominica inhibited the growth of Staphylococcus aureus at all used extract amounts (200, 150, 100 and 50 mg mL-1). S. spinosa showed no antimicrobial activity. Based on variation of RAPD patterns obtained with four primers (B-7, B-10, B-12 and B-17), the similarity among Salvia sp. was determined. The UPGMA-based (unweighted pair group method of arithmetic means) dendogram was constructed between the three species by using the similarity matrix results.