Abstract: The competitiveness of pollen tube growth in wide crosses involves Gossypium hirsutum and Gossypium arboreum was studied in six backcross entries. During the process of pollination and fertilization, physical and physiological constraints led to reduction of pollen tube growth under different levels of pistils. The differences in the number of germinating pollen grains on the stigma among the different crosses are maintained when the pollen tubes penetrate the transmitting tissue and grow along the first half of the style. The differences among entries for pollen tube growth at different levels of pistils was more significant at initial level than at later stages. A reduction in the number of pollen tubes growing down the style was recorded in all the treatment over different levels of pistils. The differences in reduction of 50.34% of pollen tube was noticed in the cross (SahanaxPA 255) xSahana while (MCU 12xPA 255) xMCU12 exhibited higher level of pollen tube proliferation. The crosses (MCU 12xPA 255) xMCU 12 exhibited significantly lower value of pollen tube proliferation at different levels of pistils. Stylar attrition was determined from the pollen tube not reaching the ovary. Stylar attrition was maximum in the cross (MCU 12xPA 255) xMCU 12 and is minimum in the cross, (SahanaxPA 255) xSahana. Though a very low pollen germination over stigmatic surface noticed in (MCU 12xPA 255) xMCU 12, lower stylar attrition of 33.53% was reported. A reduction in the number of pollen tubes as they grow along the style was observed. The genetic system of pollen pistil interaction seems to be more conspicuous in the upper part of the style than in the lower half and hence fertilization does not depend uniquely on passive physical or physiological constraints by the pistil but also on the genetic interactions among the male gametophytes and the female tissues.