Abstract: An experiment was conducted to study the effect of variety and different nursery conditions on the survivability of air-layers of two varieties of guava at the Germplasm Centre (GPC) of Fruit Tree Improvement Project, Department of Horticulture, Bangladesh Agricultural University, Mymensingh during the period from August 2001 to July 2002. Planting air-layer after detached from the mother plant and planted under different nursery conditions showed significant variation in success of air-layers. Open condition (both layers in poly bag and layers in situ) increased the percentage of survivability then under shade condition. Layers in situ (both shade and open condition) increased the number of shoots and leaves of the detached air-layers than layers in polybag. The highest percentage of survivability (100%) was observed from layers in situ under open condition which was statistically similar to layers in polybag under open condition.