Abstract: The field research was conducted at Student’s Experimental Farm, Sindh Agriculture University, Tandojam, Pakistan to assess the suitable nitrogen level for inoculated and un-inoculated soybean. The treatments 0, 50 and 75 kg N ha-1 were applied to the inoculated and un-inoculated soybean. The results of the experiment exhibited more pods per plant, maximum seeds per pod, heavier seed weight, seed index, highest nodule number and outstanding seed yield performances were observed in the plots treated with 50 kg N ha-1+inoculum (R.japonicum), followed by 75 kg N ha-1+inoculum. However, yield and yield components showed less response in the un-inoculated plots receiving no fertilizer application. The results further revealed that as the fertilizer levels increased or decreased from 50 kg N ha-1 in the inoculated plots all the observed plant characters significantly decreased. Thus, 50 kg N ha-1 and seed inoculation are recommended for achieving maximum seed yield and its contributing components.