Abstract: The field experiment was conducted at Student Farm, Sindh Agriculture University, Tandojam, Pakistan Three varieties (Early Raya, P-53/48-2 and S-9) and six NP combinations (Control, 50-15, 75-30, 100-45, 125-60 and 150-75 Kg NP ha-1) were applied to observe their effect on mustard seed yield and oil content. The results revealed highly significant differences for yield and oil content of varieties, NP levels and their interactions. NP fertilizer at the rate of 150-75 Kg ha-1 significantly increased all the crop traits. Among the varieties, S-9 produced higher seed yield whereas, oil content was higher in Early Raya.