Abstract: Some Hypericum species from Turkey were investigated for presence of hypericin and it was found in 12 of the 18 evaluated species. Hypericin is reported by us in 4 species for the first time. Most of the species contained hypericin and its total content varied widely from 0.003% in H. venustum to 0.303% in H. perforatum. The presence of hypericin in Hypericum species has an important pharmacological value for their medicinal evaluation and taxonomic value for infrageneric classification of the genus.