Abstract: A laboratory experiment was conducted at the Asian Vegetable Research and Development Center, Taiwan to assess the genetic diversity of mungbean [Vigna radiata (L.) Wilczek] cultivars using DNA markers. In this study, 21 mungbean cultivars were subjected to RAPD analysis using 34 decamer primers. All the primers produced polymorphic amplification products with some extent of variation. A total of 204 bands were generated with an average of 6.0 per primer and exhibited 75.0% polymorphism. Jaccard’s similarity coefficient ranged from 0.54 to 0.85 and concentrated mostly between 0.61 to 0.74. This indicated a rather narrow genetic base of tested cultivars. Genetic similarity obtained in this study may be used for selecting parents for breeding purposes. Clustering of cultivars into four groups showed reasonable variability that may be exploited for yield improvement.