Abstract: In cocoa cotyledon, storage albumin is abundantly present compared to other proteins. Two-dimensional electrophoresis (2-D IEF/SDS-PAGE) and native isoelectric focusing (native-IEF) techniques were used to analyse some of its biochemical characteristics. 2-D IEF/SDS-PAGE analysis by using commercially available immobilised pH gradient (IPG) gels in the first dimension for IEF exhibited reproducible patterns and high resolution of polypeptide patterns. The storage albumin (22 kDa) exhibited as a single form on 2-D IEF/SDS-PAGE with pI at around 4.6. An albumin was not resolved into multiple forms in IEF. The study found that there was similar analytical identity in storage albumin for all cultivars. This indicates that the storage albumin did not reflect genetic differences.