Abstract: Tissue culture is considered as an alternative tool for garlic breeding as traditional breeding is not possible due to its sterility. A study was carried out to develop a protocol of regeneration in Bangladesh local garlic. Shoot and root meristems of garlic cv. Bangladesh local were cultured on Murashige and Skoog (MS) medium containing various growth regulator combinations. Regeneration from shoot meristem was found with all medium composition but highest (95.55%) in growth regulator-free medium. Shoots were transferred onto media containing BA or high level of sucrose. Forty per cent (40.00%) of the root meristem regenerated shoots on MS medium supplemented with 1 µM NAA and 10 µM BA. The shoots were multiplied on MS medium containing 0.5 µM BA. Bulblets were obtained from the regenerated shoots of garlic in all the media. It is the first to report regeneration from any Bangladeshi garlic.