Abstract: The present study was carried out to clone and sequence 1-aminocyclopropane-1-carboxylate synthase (ACC synthase, pBA-ACS) from a sliced moso bamboo shoot using reverse transcription and polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends-PCR (RACE-PCR). The cDNA clone of pBA-ACS was 1793 bp in length and contained a 5'-untranslated region of 126 bp, an open reading frame of 1446 bp encoding 482 amino acids and 3'-untranslated region of 221bp containing stop codone. The pBA-ACS was highly homologous to ACC synthase genes from rice, followed by apple and arabidopsis. In northern analysis, expression of pBA-ACS mRNA was enhanced in wounding tissue until 24 h and coincided with the peak of ACS synthase activity but disagreed with ethylene production. The ACC synthase activity suggested that the increase might be the response to the wounding associated with harvest.