Abstract: The high molecular weight (HMW) glutenin subunits from seven Pakistani wheat genotypes were fractionated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), in order to characterise the plant material and test the variability within species. The mitotic results indicated that the chromosomal count in six genotypes was 2n= 42. The number of chromosomes in variety Sargab-92 were somewhat irregular, showing 2n= 40 as well as 41 instead of 42 chromosomes. The banding patterns of HMW glutenin subunits of 6 Pakistani wheat genotypes were proved to be similar and a genetic variability was shown by the genotype Sargab-92, in which 4 extra bands had been identified.