Abstract: The research was conducted to investigate the comparative resistance of nine maize cultivars/lines i.e. W-2, FRY-4, EV-9808, FRY-1, EV-9806, W-19, Ghori, Pahari and Babar, to maize stem borer, Chilo partellus (Swinhoe). The lowest significant infestation was recorded on EV-9806 (5.29%), EV-9808 (6.21%) and FRY-1 (6.32%), while the lowest number of dead hearts were observed on FRY-1 (4.10%), EV-9808 (5.40%), Pahari (5.47%) and W-2 (5.51%), throughout the growing season. The result based on these parameters revealed that line FRY-1 was comparatively the most resistant followed by EV-9808, Pahari and W-2. Overall result can be summed up for maize cultivars/lines resistance to maize stem borer FRY-1 > EV-9808 > Pahari> W-2 > W-19.